Optimisation of Omega 3 Rich Oil extraction from elateriospermum tapos seed by microwave assisted aqueous enzymatic extraction

Abstract

Microwave assisted aqueous enzymatic extraction (MAAEE) of Elateriospermum Tapos Seed (ETS) was performed in this study. This is a novel method which can reduce the extraction time and solvent consumption. The Elateriospermum Tapos oil (ETO) yield was optimised by using a central composite design (CCD) at condition parameters of microwave power (110 - 1,100 W), extraction time (30 - 120 s), enzyme cocktail concentration (1 - 5 %) and particle size (0.5 - 1.5 mm). The effect of each parameter was investigated by Response Surface Methodology (RSM). The optimal oil extraction yield was obtained at 110 W microwave power, 30 s extraction time, 1 % enzyme cocktail concentration and 0.5 mm particle size. Under these conditions, the maximum value obtained for the oil extraction yield was 46.12 ± 1.48 % recovery which was in good agreement with the predicted value (43.87 %). Thus, MAAEE could speed up extraction process as compared to Soxhlet which required 6 h extraction time to achieve similar amount of extraction yield.