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Kinetic study on the hydrolysis of palm olein using immobilized lipase

Chew, Yin Hoon and Chua, Lee Suan and Cheng , Kian Kai and Sarmidi, Mohamad Roji and Abdul Aziz, Ramlan and Lee, Chew Tin (2008) Kinetic study on the hydrolysis of palm olein using immobilized lipase. Biochemical Engineering Journal, 39 (3). pp. 516-520. ISSN 1369-703X

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Official URL: http://dx.doi.org/10.1016/j.bej.2007.10.019

Abstract

The application of immobilized lipase (EC 3.1.1.3) is gaining interest in the oleochemical industry as it offers advantages over conventional chemical reactions. In the present study, a commercial immobilized lipase, Lipozyme TL IM, was used to catalyze the hydrolysis of palm olein in an aqueous-organic phase. A ping-pong bi-bi model with substrate inhibition by water was used to describe the hydrolysis reaction. The reaction rate constants of the proposed mechanism were determined by fitting the model into experimental data using a nonlinear curve fitting software. Based on the results of this study, the model proposed was able to fit the data with a correlation coefficient of 0.9586. The rate of formation of fatty acids is limited by the formation of glycerol. The critical water content before inhibition occurs was found to be 3.6% (v/v). No inhibition by palm olein was observed up to a concentration of 874.76 g/l.

Item Type:Article
Uncontrolled Keywords:Aqueous two phase, heterogeneous reactions, hydrolysis, immobilized enzymes, lipase, modeling
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:7565
Deposited By: Norhafizah Hussin
Deposited On:09 Jan 2009 01:23
Last Modified:01 Jun 2010 15:53

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