Ali Sidahmed, Heyam Mohamed and Mohd. Hashim, Najihah and Mohan, Syam Morali and Abdelwahab, Siddig Ibrahim and Taha, Manal Mohamed Elhassan and Dehghan, Firouzeh and Yahayu, Maizatulakmal Akmal and Cheng, Gwendoline Lian Ee and Loke, Mun Fai and Vadivelu, Jamuna (2016) Evidence of the gastroprotective and anti-helicobacter pylori activities of β-mangostin isolated from Cratoxylum arborescens (Vahl) blume. Drug Design, Development and Therapy, 10 . pp. 297-313. ISSN 1177-8881
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Abstract
β-Mangostin (BM) from Cratoxylum arborescens demonstrated various pharmacological activities such as anticancer and anti-inflammatory. In this study, we aimed to investigate its antiulcer activity against ethanol ulcer model in rats. Materials and methods: BM was isolated from C. arborescens. Gastric acid output, ulcer index, gross evaluation, mucus production, histological evaluation using hematoxylin and eosin and periodic acid–Schiff staining and immunohistochemical localization for heat shock protein 70 (HSP70) and Bax proteins were investigated. Possible involvement of reduced glutathione, lipid peroxidation, prostaglandin E2, antioxidant enzymes, superoxide dismutase and catalase enzymes, radical scavenging, nonprotein sulfhydryl compounds, and anti-Helicobacter pylori were investigated. Results: BM showed antisecretory activity against the pylorus ligature model. The pretreatment with BM protect gastric mucosa from ethanol damaging effect as seen by the improved gross and histological appearance. BM significantly reduced the ulcer area formation, the submucosal edema, and the leukocytes infiltration compared to the ulcer control. The compound showed intense periodic acid–Schiff staining to the gastric mucus layer and marked amount of alcian blue binding to free gastric mucus. BM significantly increased the gastric homogenate content of prostaglandin E2 glutathione, superoxide dismutase, catalase, and nonprotein sulfhydryl compounds. The compound inhibited the lipid peroxidation revealed by the reduced gastric content of malondialdehyde. Moreover, BM upregulate HSP70 expression and downregulate Bax expression. Furthermore, the compound showed interesting anti-H. pylori activity. Conclusion: Thus, it could be concluded that BM possesses gastroprotective activity, which could be attributed to the antisecretory, mucus production, antioxidant, HSP70, antiapoptotic, and anti-H. pylori mechanisms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | alcian blue, alcohol, antiinfective agent, beta mangostin, catalase, Cratoxylum arborescens extract, eosin, glutathione, heat shock protein 70, hematoxylin, omeprazole, plant extract, prostaglandin E2, protein Bax, superoxide dismutase, unclassified drug, alcohol, antiulcer agent, heat shock protein 70, malonaldehyde, mangostin, protein Bax, xanthone derivative, animal experiment, animal model, antibacterial activity, Article, controlled study, Cratoxylum arborescens, down regulation, drug isolation, female, Helicobacter pylori, Hypericaceae, immunohistochemistry, in vivo study, leukocyte, lipid peroxidation, male, nonhuman, periodic acid Schiff stain, protein expression, pylorus ligation induced gastric ulcer, rat, stomach content, stomach protection, animal, apoptosis, chemistry, Clusiaceae, disease model, drug effects, genetics, isolation and purification, metabolism, pathology, Sprague Dawley rat, stomach mucosa, stomach ulcer, upregulation, Animals, Anti-Ulcer Agents, Apoptosis, bcl-2-Associated X Protein, Clusiaceae, Disease Models, Animal, Down-Regulation, Ethanol, Female, Gastric Mucosa, Helicobacter pylori, HSP70 Heat-Shock Proteins, Lipid Peroxidation, Male, Malondialdehyde, Rats, Rats, Sprague-Dawley, Stomach Ulcer, Up-Regulation, Xanthones |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Chemical Engineering |
| ID Code: | 73975 |
| Deposited By: | Fahmi Moksen |
| Deposited On: | 23 Nov 2017 05:09 |
| Last Modified: | 23 Nov 2017 05:09 |
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