Shukor, Hafiza and Abdeshahian, Peyman and Al-Shorgani, Najeeb Kaid Nasser and Abdul Hamid, Aidil and A. Rahman, Norliza and Kalil, Mohd. Sahaid (2016) Saccharification of polysaccharide content of palm kernel cake using enzymatic catalysis for production of biobutanol in acetone-butanol-ethanol fermentation. Bioresource Technology, 202 . pp. 206-213. ISSN 0960-8524
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Abstract
In this work, hydrolysis of cellulose and hemicellulose content of palm kernel cake (PKC) by different types of hydrolytic enzymes was studied to evaluate monomeric sugars released for production of biobutanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in acetone-butanol-ethanol (ABE) fermentation. Experimental results revealed that when PKC was hydrolyzed by mixed β-glucosidase, cellulase and mannanase, a total simple sugars of 87.81 ± 4.78 g/L were produced, which resulted in 3.75 ± 0.18 g/L butanol and 6.44 ± 0.43 g/L ABE at 168 h fermentation. In order to increase saccharolytic efficiency of enzymatic treatment, PKC was pretreated by liquid hot water before performing enzymatic hydrolysis. Test results showed that total reducing sugars were enhanced to 97.81 ± 1.29 g/L with elevated production of butanol and ABE up to 4.15 ± 1.18 and 7.12 ± 2.06 g/L, respectively which represented an A:B:E ratio of 7:11:1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Acetone, Cellulose, Clostridium, Enzymes, Ethanol, Fermentation, Hydrolysis, Saccharification, Sugars, Acetone-butanol-ethanol fermentation, Cellulose and hemicellulose, Clostridium saccharoperbutylacetonicum n1-4, Enzyme treatment, Fermentable sugars, Palm kernel cake, Polysaccharide contents, Total reducing sugars, Enzymatic hydrolysis, acetone, alcohol, beta glucosidase, beta mannosidase, biobutanol, biofuel, butanol, cellulase, cellulose, glucose, hemicellulose, hot water, mannose, polysaccharide, unclassified drug, xylose, acetone, alcohol, butanol, polysaccharide, solid waste, sucrose, biofuel, catalysis, cellulose, enzyme activity, ethanol, experimental study, fermentation, hydrolysis, polysaccharide, Arecaceae, Article, biofuel production, catalysis, Clostridium, Clostridium saccharoperbutylacetonicum, fermentation, hydrolysis, liquid, nonhuman, nut kernel, palm kernel cake, priority journal, saccharification, Arecaceae, carbohydrate metabolism, catalysis, chemistry, fermentation, metabolism, solid waste, Butanols, Catalysts, Enzymatic Activity, Fermentation, Hemicelluloses, Polysaccharides, Sugars, Clostridium saccharoperbutylacetonicum, 1-Butanol, Acetone, Arecaceae, beta-Glucosidase, Butanols, Carbohydrate Metabolism, Catalysis, Cellulase, Cellulose, Clostridium, Ethanol, Fermentation, Hydrolysis, Polysaccharides, Solid Waste, Sucrose |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Chemical Engineering |
| ID Code: | 73931 |
| Deposited By: | Fahmi Moksen |
| Deposited On: | 22 Nov 2017 12:07 |
| Last Modified: | 22 Nov 2017 12:07 |
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