Screening for ligninolytic enzyme production by white rot fungi from Malaysia using different medium

Abstract

White rot fungi were extensively reviewed as one for the groups instituted as the most efficient lignin degrader in nature. However, the induction of ligninolytic production varies upon different compositions in medium used for cultivation. In the present work we report the production of ligninolytic enzymes by two white rot fungi under different medium cultivation. Those strains produced significant amounts of Lignin Peroxidase, Manganese Peroxidase and Laccase enzymes in medium containing lignin were further employed to the various medium screening studies. The predominant ligninolytic producers identified as Cerrena sp. WICC F39 and P. chrysosporium WICC F38 found to be capable of producing consortium of ligninolytic enzymes mainly laccase in higher titres than P. chrysosporium DSMZ 6909. Cerrena sp. WICC F39 was the best producers of manganese peroxidase and laccase enzymes and the secretion were most enhanced using medium 4. A medium containing 10 g L-1 glucose, 3.5 g L-1 D-glucose, 0.054 g L-1 NH4Cl, 0.136 KH2PO, 0.044 g L-1 K2SO4, 0.775 MgCl2.6H2O 0.775, 0.1 g L-1 CaCl2.2H2O, 300 g L-1 thiamine-HCl and 0.1 ml trace element solution proved optimum for the enzymatic processes. High titres of laccase and significant amount of lignin and manganese peroxidase were observed at 56.34±6.37 U L-1, 2.05±0.27 U L-1 and 3.78±0.02 U L-1. Depletion of laccase and low enzymes activities for manganese peroxidase and lignin peroxidase were observed for Phanerochaete WICC F39 as compared to standard strains P. chrysosporium DSMZ. Both local isolates, Phanerochaete sp. WICC F38 and Cerrena sp. WICC F39 were deposited to The National Center for Biotechnology Information (NCBI) with accession no: KU141330.1 and KU 141331.1.