Woon, J. S. K. and Mackeen, M. M. and Sudin, A. H. and Mahadi, N. M. and Illias, R. M. and Murad, A. M. A. and Bakar, F. D. A. (2016) Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris. Biotechnology Letters, 38 (5). pp. 825-832. ISSN 0141-5492
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Abstract
Objectives: To express and determine the hydrolytic activity of a cellobiohydrolase (TTCBH6B) from the thermophilic fungus Thielavia terrestris in Pichia pastoris. Results: Ttcbh6B encodes a protein of 507 amino acid residues with a predicted molecular mass of 54 kDa. TTCBH6B contains a familial 6-glycosyl hydrolase catalytic domain and a type I carbohydrate-binding module. TTCBH6B was expressed and purified to homogeneity but the purified enzyme was inactive against Avicel. It could, however, digest Celluclast-treated Avicel producing cellobiose (0.27 μmol min−1 mg−1). To determine the substrate preferences of TTCBH6B, oligosaccharides of varying numbers of subunits were generated by acid hydrolysis of Avicel and fluorescently tagged. Peaks corresponding to oligosaccharides containing three to six glucose units were reduced to cellobiose after addition of TTCBH6B. Conclusion: TTCBH6B is active against shorter oligosaccharides rather than polysaccharides.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Bins, Carbohydrates, Enzymes, Fungi, Hydrolases, Purification, 2-Aminobenzamide, Avicel, Carbohydrate binding modules, Cellobiose, Celluclast, Endoglucanases, Glycosyl hydrolases, Oligosaccharides, cellobiose, cellulose 1,4 beta cellobiosidase, oligosaccharide, recombinant protein, chemistry, enzyme specificity, enzymology, genetics, isolation and purification, metabolism, molecular weight, Pichia, Sordariales, Cellobiose, Cellulose 1,4-beta-Cellobiosidase, Molecular Weight, Oligosaccharides, Pichia, Recombinant Proteins, Sordariales, Substrate Specificity |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Chemical Engineering |
| ID Code: | 72591 |
| Deposited By: | Haliza Zainal |
| Deposited On: | 27 Nov 2017 08:13 |
| Last Modified: | 27 Nov 2017 08:13 |
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