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Production of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. TS1-1: Media optimization using experimental design

Mahat, Mohd Khairizal and Illias, Rosli Md. and Rahman, Roshanida A. and Rashid, Noor Aini Abd and Mahmood, Nik Azmi Nik and Hassan, Osman and Aziz, Suraini Abdul and Kamaruddin, Kamarulzaman (2004) Production of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. TS1-1: Media optimization using experimental design. Enzyme and Microbial Technology, 35 (5). 467-473 . ISSN 0141-0229

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Official URL: http://dx.doi.org/10.1016/j.enzmictec.2004.07.008

Abstract

Cyclodextrin glucanotransferase (CGTase) was produced when the Bacillus sp. TS1-1 was grown in a medium containing sago starch, yeast extract, phosphorus and mineral salt sources, using shake flask mode at 37°C for 24 h. Response surface methodology (RSM) was applied to optimize the medium constituents with respect to CGTase production and activity. A 24 full factorial design (first order model) was carried out to identify the significant effect of medium components towards CGTase production. The variables involved in this initial screening study were sago starch, yeast extract, K2HPO 4 and MgSO4·7H2O. Statistical analysis of results have shown that only sago starch and yeast extract have a significant effect on CGTase production. A second-order model was proposed by using 2 2 central composite design to represent the production CGTase activity as a function of sago starch and yeast extract. The optimized values of 1.48% and 1.89% of sago starch and yeast extract was obtained, respectively. Under these proposed optimized conditions, the model predicted a CGTase activity of 79.66 U/ml and via experimental rechecking the model, an activity of 84 U/ml was attained.

Item Type:Article
Uncontrolled Keywords:Cyclodextrin, cyclodextrin glucanotransferase, medium optimization, response surface methodology
Subjects:Q Science > QR Microbiology
Divisions:Science
ID Code:7239
Deposited By: Maznira Sylvia Azra Mansor
Deposited On:01 Jan 2009 04:41
Last Modified:01 Jun 2010 15:50

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