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Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor

Phuah, E. T. and Lee, Y. Y. and Tang, T. K. and Lai, O. M. and Choong, T. S. Y. and Tan, C. P. and Ng, W. N. and Lo, S. K. (2016) Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor. International Journal of Food Engineering, 12 (7). pp. 681-689. ISSN 2194-5764

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Abstract

Response surface methodology (RSM) was employed to optimize the process variables namely packed bed height (cm) and flow rates (ml/min) on diacylglycerol (DAG) production via partial hydrolysis of palm oil using immobilized Rhizomucor miehei lipase in packed bed reactor (PBR). Quadratic models were successfully developed for both DAG(y) and unhydrolyzed triacylglycerol ((un)TAG) with determination coefficient (R2) of 0.9931 and 0.9986, respectively coupled with insignificant lack of fit (p > 0.05). Optimal conditions for DAG synthesis were evaluated to be 10cm packed bed height and 3.8ml/min flow rate. Immobilized enzyme can be reused up to 10 times without significant changes in enzymatic activity. The partial hydrolysis under studied was found to be mass transfer-controlled.

Item Type:Article
Uncontrolled Keywords:Chemical reactors, Enzymes, Glycerol, Hydrolysis, Isomers, Lipids, Mass transfer, Oil shale, Palm oil, Surface properties, Determination coefficients, Diacylglycerol, Enzymatic activities, Modeling and optimization, Packed bed reactor, Partial hydrolysis, Response surface methodology, Rhizomucor miehei lipase, Packed beds
Subjects:L Education > L Education (General)
Divisions:Education
ID Code:72138
Deposited By: Fazli Masari
Deposited On:21 Nov 2017 03:28
Last Modified:21 Nov 2017 03:28

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