Mienda, B.S. and Shamsir, M.S. and Md. Illias, R. (2016) Model-assisted formate dehydrogenase-O (fdoH) gene knockout for enhanced succinate production in Escherichia coli from glucose and glycerol carbon sources. Journal of Biomolecular Structure and Dynamics, 34 (11). pp. 2305-2316. ISSN 0739-1102
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Abstract
Succinic acid is an important platform chemical that has broad applications and is been listed as one of the top twelve bio-based chemicals produced from biomass by the US Department of Energy. The metabolic role of Escherichia coli formate dehydrogenase-O (fdoH) under anaerobic conditions in relation to succinic acid production remained largely unspecified. Herein we report, what are to our knowledge, the first metabolic fdoH gene knockout that have enhanced succinate production using glucose and glycerol substrates in E. coli. Using the most recent E. coli reconstruction iJO1366, we engineered its host metabolism to enhance the anaerobic succinate production by deleting the fdoH gene, which blocked H+ conduction across the mutant cell membrane for the enhanced succinate production. The engineered mutant strain BMS4 showed succinate production of 2.05 g l−1 (41.2-fold in 7 days) from glycerol and.39 g l−1 (6.2-fold in 1 day) from glucose. This work revealed that a single deletion of the fdoH gene is sufficient to increase succinate production in E. coli from both glucose and glycerol substrates.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | formate dehydrogenase, glucose, glycerol, reduced nicotinamide adenine dinucleotide, succinic acid, formate dehydrogenase, formate dehydrogenase-O, glucose, glycerol, succinic acid, anaerobic fermentation, Article, bacterial gene, bacterial metabolism, bacterial strain, carbon source, cell membrane, cell mutant, computer model, concentration (parameters), controlled study, electroporation, Escherichia coli, fdoH gene, gene deletion, gene inactivation, host, nonhuman, priority journal, biology, Escherichia coli, gene inactivation, genetics, metabolism, Computational Biology, Escherichia coli, Formate Dehydrogenases, Gene Knockout Techniques, Glucose, Glycerol, Metabolic Networks and Pathways, Sequence Deletion, Succinic Acid |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Biosciences and Medical Engineering |
| ID Code: | 71953 |
| Deposited By: | Fazli Masari |
| Deposited On: | 22 Nov 2017 12:07 |
| Last Modified: | 22 Nov 2017 12:07 |
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