Atago, Y. and Shimodaira, J. and Araki, N. and Othman, N. B. and Zakaria, Z. and Fukuda, M. and Futami, J. and Hara, H. (2016) Identification of novel extracellular protein for PCB/biphenyl metabolism in Rhodococcus jostii RHA1. Bioscience, Biotechnology and Biochemistry, 80 (5). pp. 1012-1019. ISSN 0916-8451
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Abstract
Rhodococcus jostii RHA1 (RHA1) degrades polychlorinated biphenyl (PCB) via co-metabolism with biphenyl. To identify the novel open reading frames (ORFs) that contribute to PCB/biphenyl metabolism in RHA1, we compared chromatin immunoprecipitation chip and transcriptomic data. Six novel ORFs involved in PCB/biphenyl metabolism were identified. Gene deletion mutants of these 6 ORFs were made and were tested for their ability to grow on biphenyl. Interestingly, only the ro10225 deletion mutant showed deficient growth on biphenyl. Analysis of Ro10225 protein function showed that growth of the ro10225 deletion mutant on biphenyl was recovered when exogenous recombinant Ro10225 protein was added to the culture medium. Although Ro10225 protein has no putative secretion signal sequence, partially degraded Ro10225 protein was detected in conditioned medium from wild-type RHA1 grown on biphenyl. This Ro10225 fragment appeared to form a complex with another PCB/biphenyl oxidation enzyme. These results indicated that Ro10225 protein is essential for the formation of the PCB/biphenyl dioxygenase complex in RHA1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Biodegradation, Metabolism, Physiology, Polychlorinated biphenyls, Proteins, Recombinant proteins, Biphenyl, Chromatin immunoprecipitation, Extracellular proteins, Gene deletion mutants, Open reading frame, Polychlorinated biphenyls (PCB), RHA1, Rhodococcus, Organic pollutants, bacterial protein, biphenyl, biphenyl derivative, conditioned medium, dioxygenase, polychlorinated biphenyl, recombinant protein, transcriptome, amino acid sequence, chemistry, chromatin immunoprecipitation, conditioned medium, drug effects, enzymology, Escherichia coli, gene deletion, gene expression profiling, gene expression regulation, genetic complementation, genetics, metabolism, molecular cloning, molecular genetics, open reading frame, Rhodococcus, Amino Acid Sequence, Bacterial Proteins, Biphenyl Compounds, Chromatin Immunoprecipitation, Cloning, Molecular, Culture Media, Conditioned, Dioxygenases, Escherichia coli, Gene Deletion, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Genetic Complementation Test, Molecular Sequence Annotation, Open Reading Frames, Polychlorinated Biphenyls, Recombinant Proteins, Rhodococcus, Transcriptome |
| Subjects: | T Technology > T Technology (General) |
| Divisions: | Malaysia-Japan International Institute of Technology |
| ID Code: | 71725 |
| Deposited By: | Widya Wahid |
| Deposited On: | 20 Nov 2017 08:46 |
| Last Modified: | 20 Nov 2017 08:46 |
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