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Determination of carotene, tocopherols and tocotrienols in residue oil from palm pressed fiber using pressurized liquid extraction-normal phase liquid chromatography

Sanagi, M. Marsin and See, H.H. and Wan Ibrahim, Wan Aini and Abu Naim, Ahmedy (2005) Determination of carotene, tocopherols and tocotrienols in residue oil from palm pressed fiber using pressurized liquid extraction-normal phase liquid chromatography. Analytica Chimica Acta, 538 (71-76). ISSN 0003-2670

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Official URL: http://dx.doi.org/10.1016/j.aca.2005.02.028

Abstract

Pressurized liquid extraction (PLE) along with normal phase liquid chromatography (NPLC) is evaluated as a new approach for the determination of -carotene and Vitamin E isomers in residue oil obtained from palm pressed fiber (PPF). The relative extraction recoveries obtained by optimized PLE were found to be equivalent (~100%) to conventional Soxhlet extraction. Optimized PLE was obtained (5 g of sample, 80 ◦C, 1500 psi, 2× 10 min static extractions with flush volume 50%) combined with NPLC system. Typical extractions give 3.7–4.0 mg ml-1 of Vitamin E isomers and -carotene of 3.3–3.5 mg ml-1, which is similar to or greater than classical Soxhlet extraction with n-hexane or chloroform. The new developed method demonstrated an acceptable performance with good efficiency in terms of total extraction time, total solvent usage, total -carotene and Vitamin E isomers contents as well as exceptional method repeatability. © 2005 Published by Elsevier B.V.

Item Type:Article
Uncontrolled Keywords:pressurized liquid extraction, liquid chromatography, tocopherol, tocotrienol, palm oil
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:7106
Deposited By: Marlina Kasiman
Deposited On:18 Dec 2008 03:46
Last Modified:01 Jun 2010 15:49

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