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Novel gold nanoparticles reduced by sargassum glaucescens: preparation, characterization and anticancer activity

Ajdari, Zahra and Rahman, Heshu and Shameli, Kamyar and Abdullah, Rasedee and Abd. Ghani, Maaruf and Yeap, Swee and Abbasiliasi, Sahar and Ajdari, Daniel and Ariff, Arbakariya (2016) Novel gold nanoparticles reduced by sargassum glaucescens: preparation, characterization and anticancer activity. Molecules, 21 (3). pp. 1-17. ISSN 1420-3049

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Official URL: http://dx.doi.org/10.3390/molecules21030123

Abstract

The current study investigated the anticancer properties of gold nanoparticles (SG-stabilized AuNPs) synthesized using water extracts of the brown seaweed Sargassum glaucescens (SG). SG-stabilized AuNPs were characterized by ultraviolet-visible spectroscopy, transmission and scanning electron microscopy, and energy dispersive X-ray fluorescence spectrometry. The SG-stabilized AuNPs were stable and small at 3.65 ± 1.69 nm in size. The in vitro anticancer effect of SG-stabilized AuNPs was determined on cervical (HeLa), liver (HepG2), breast (MDA-MB-231) and leukemia (CEM-ss) cell lines using fluorescence microscopy, flow cytometry, caspase activity determination, and MTT assays. After 72 h treatment, SG-stabilized AuNPs was shown to be significant (p < 0.05) cytotoxic to the cancer cells in a dose- and time-dependent manner. The IC50 values of SG-stabilized AuNPs on the HeLa, HepG2, CEM-ss, MDA-MB-231 cell lines were 4.75 ± 1.23, 7.14 ± 1.45, 10.32 ± 1.5, and 11.82 ± 0.9 µg/mL, respectively. On the other hand, SG-stabilized AuNPs showed no cytotoxic effect towards the normal human mammary epithelial cells (MCF-10A). SG-stabilized AuNPs significantly (p < 0.05) arrest HeLa cell cycle at G2/M phase and significantly (p < 0.05) activated caspases-3 and -9 activities. The anticancer effect of SG-stabilized AuNPs is via the intrinsic apoptotic pathway. The study showed that SG-stabilized AuNPs is a good candidate to be developed into a chemotherapeutic compound for the treatment of cancers especially cervical cancer.

Item Type:Article
Uncontrolled Keywords:anticancer properties, cervical cancer, gold nanoparticles
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:70067
Deposited By: Narimah Nawil
Deposited On:22 Nov 2017 00:45
Last Modified:22 Nov 2017 00:45

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