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Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers

Halimoon, H. and Hussain, A. R. and Kouzani, A. and Alam, M. N. H. Z. (2016) Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers. Sains Malaysiana, 45 (6). pp. 969-976.

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Official URL: http://www.ukm.my/jsm/pdf_files/SM-PDF-45-6-2016/1...

Abstract

In this paper, a minibioreactor platform made of low cost polymers is presented. The minibioreactor prototype was designed as an alternative solution for carrying out microbial fermentation experiments in laboratory. The minibioreactor prototype has a working volume of 1.5 mL and was fabricated from poly(methylmethacrylate) ( PMMA ) and poly(dimethylsiloxane) ( PDMS ) polymers. Cell density was measured online whilst agitation rates and the temperature of the reactor content can be tightly controlled to desired set-point values. As proof-of-concept, various S. cerevisae fermentation experiments were conducted. In every experiment, the minibioreactor operated stably for the entire length of operation which was nearly 40 h with very minimal volume loss i.e. about 2.8 μ L·h -1 at 37 º C. The minibioreactor has the maximum oxygen transfer rate ( OTR ) of 16.6 mmol·L -1 ·h -1 under the agitation rate of 300 rpm. Under these conditions, cell specific growth rate as high as 0.291 h -1 was obtained. The experimental data in the minibioreactor operation was also reproducible using shake flask where similar growth profiles were attained under a similar growth conditions.

Item Type:Article
Subjects:T Technology > TP Chemical technology
Divisions:Chemical Engineering
ID Code:68756
Deposited By: Haliza Zainal
Deposited On:06 Nov 2017 05:26
Last Modified:20 Nov 2017 08:52

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