Application of EZ–Enzyme in Bioremediation of Oily Sludge

Jenny Ho, Mohd. Rashid Mohd. Yusof

Abstract



Sludge farming adalah cara biasa yang digunakan dalam loji penapisan petroleum untuk merawat sisa minyak yang mengandungi bahan berupaya terdegradasi kepada bahan yang tidak toksid terhadap kesihatan manusia dan alam sekitar. Sisa tersebut akan dibiarkan di atas tanah supaya terdegradasi secara semula jadi tetapi proses ini akan mengambil masa yang lama. Dalam kajian ini, sejenis enzim bernama Enzim EZ telah diuji untuk bioremediasi sampel sisa minyak dalam uji kaji yang berskala makmal. Keputusan menunjukkan bahawa terdapat pengurangan secara konsisten untuk kandungan minyak dan gris dalam sampel yang diuji (secara rata ~ 60%). Kandungan tersebut berkurangan dengan meningkatnya kepekatan Enzim EZ. Kandungan minyak dan gris terdegradasi dengan paling banyak dalam sampel mengandungi 100 ppm Enzim EZ iaitu dari 8% ke 2% dalam eksperimen selama sembilan minggu. Secara umum, suhu dan pH dikekalkan pada keadaan optimum, iaitu 35°C dan pH 7. Kualiti air serapan yang dihasilkan juga telah menunjukkan peningkatan kualiti di akhir eksperimen, iaitu keperluan oksigen kimia (COD) ialah 25 mg/l dan pH ialah 6.45.

Kata kunci: Bioremediasi, enzim, kandungan minyak dan gris, sludge farming

Sludge farming is commonly used in petroleum refinery as a method of treatment for oily waste containing degradable constituents into materials that are not hazardous to human health or the environment. The sludge is left on a designated plot of land to be biodegraded naturally, but the process could take a considerable length of time. In this study, an enzyme known as EZ-Enzyme was tested to bioremediate the oily sludge sample in a laboratory scale experiment. Results shown that there was a consistent reduction in the oil and grease (O&G) content of the tested sludge (~ 60% in average). The O&G content decreased with increase in the concentration of enzyme. The O&G content in a sludge sample decreased the most with the application of 100 pm EZ-Enzyme, which decreased from 8% to 2% within a period of nine weeks. In general, the temperature and pH of the system were maintained at optimum condition, which were 35°C and pH 7 respectively. The quality of the leachate was also improved by the end of the study, where chemical oxygen demand (COD) was 25 mg/l and pH was 6.45 respectively.

Key words: Bioremediation, enzyme, oil and grease content, sludge farming

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DOI: http://dx.doi.org/10.11113/jt.v48.231

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