Universiti Teknologi Malaysia Institutional Repository

Microcystin degradation in sphingopyxis sp. C-1

Sugiura, Norio and Maseda, Hideaki and Uwata, Maki and Shimizu, Kazuya and Okano, Kunihiro and Utsumi, Motoo and Iwamoto, Koji and Goto, Masafumi and Itayama, Tomoaki (2015) Microcystin degradation in sphingopyxis sp. C-1. In: International Conference on Sustainability Initiatives (ICSI 2015) in conjunction with 8th ASEAN Environmental Engineering Conference (AEEC), 24-25 August, 2015, Kuala Lumpur, Malaysia.

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Official URL: http://mjiit.utm.my/icsi2015/

Abstract

The microcystin-degrading gene cluster, mlrA-B-C-D, plaies an important role in the degradation process of hepatotoxic microcystins for several bacterial species. However after microcystin is degraded to linear-microcystin by MlrA, it is still unknown about where and by what it is metabolited. In order to clarify it, we disrupted the mlrB gene and mlrC gene in chromosome of microcystin-degrading bacteria, Sphingopyxis sp. C-1. The cells disrupted mlrB gene and mlrC gene accumulated of microcystin-degradation product, linear-microcystin and tetrapeptide, respectively, whereas the cell free extracts of ?mlrB cells detected Adda and ?mlrC cells accumulated tetrapeptide. Moreover, topology analysis of MlrB using the ß-lactamase gene fusion method insisted MlrB is the peripheral protein binding the inner-membrane. These results insist that MlrB degrades the linear microcystin in the periplasmic space and MlrC degrades tetrapeptide in cytoplasm. Thus, in intact cells, MlrC cannot degrade linear-microcystin as being separated in inner-membrane from linear-microcystin while MlrC is capable of degrading the linear-microcystin in cell-free extract.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:microcystin, water treatment
Subjects:T Technology > T Technology (General)
Divisions:Malaysia-Japan International Institute of Technology
ID Code:63373
Deposited By: Fazli Masari
Deposited On:30 May 2017 03:09
Last Modified:30 May 2017 03:09

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