Sani, N. S. and Lee, S. L. and Hamdan, H. (2008) Degradation of waste in space using alcohol-free enzyme encapsulated silica aerogel. In: 26th International Symposium on Space Technology and Science (ISTS), 1-6 June 2008, Japan.
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Official URL: http://www.ists.or.jp/2008
Enzyme (tyrosinase) is successfully encapsulated into nano-porous silica aerogel via an alcohol-free colloidal sol-gel route using organic-silica source. The encapsulation process was carried out at room temperature and at neutral pH to further minimize denaturation of enzymes. The leaching test showed that no significant enzymatic activity was observed in the filtrate of tyrosinase encapsulated nano-porous silica aerogel, which indicated that most of the enzyme was effectively loaded. The optimization study exhibited that 500 U enzyme/g aerogel which was aged for 2 days gives the superior performance in the oxidation of catechol. The enzymatic activity of tyrosinase encapsulated nano-porous silica aerogel which was assayed through reduction of ascorbic acid using UV-Vis spectrophotometer reveals remarkable enhancement which is active at wider temperature and pH range. At pH 4 and pH 9, tyrosinase encapsulated nano-porous silica aerogel oxides catechol (phenol substitute) up to 30% after 60 minutes of reaction and it retains its enzymatic activity at 80 oC. In contrast, free tyrosinase was totally inactive at these pH values and at temperature exceeding 55 oC. This indicates that the stability of the tyrosinase towards extreme temperature as well as acidic and basic conditions is significantly improved with good reusability after the encapsulation process. Thus, the combinations of enzyme, the useful protein in waste degradation system, and aerogel, the lightest solid with high mechanical strength; could to be used to decompose waste in space efficiently
|Item Type:||Conference or Workshop Item (Paper)|
|Uncontrolled Keywords:||waste degradation system, aerogel|
|Subjects:||Q Science > QD Chemistry|
|Deposited By:||Dr Siew Ling Lee|
|Deposited On:||08 Oct 2008 04:19|
|Last Modified:||07 Feb 2017 08:00|
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