Effects of formulation parameters on particle size and polydispersity index of orthosiphon stamineus loaded nanostructured lipid carrier

Abstract

This study was conducted to investigate the effect of particle size and polydispersity index (PDI) by changing the concentration of active ingredient and solid lipid in the Orthosiphon stamineus (O. stamineus) loaded nanostructured lipid carrier (NLC) formulation. O. stamineus extract was prepared by maceration method. From the HPLC analysis, the O. stamineus extract contains 38% sinensetin and 62% rosmarinic acid. The method used to prepare the formulation of O. Stamineus loaded NLC is melt emulsification homogenization technique. Solid and liquid lipid used were glyceryl monostearate and triglyceride respectively. It was found that the size of particles increased as increasing in concentration of active and solid lipid in the formulation. The best range for concentration of active and solid lipid are 1-4% and 1-3% respectively since the particle size and PDI needed are below 200 d.nm and 0.2. Collectively, based on particle size and PDI results show that the NLC system is highly potential to be a carrier of transdermal delivery for O. stamineus.