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Optimisation of Indole-3-Butyric Acid (IBA) concentrations for production of biomass and phenolics from adventitious roots of Justicia Gendarussa

Ayob, Zahidah and Abd. Samad, Azman and Mohd. Bohari, Siti Pauliena and Jamil, Shajarahtunnur (2015) Optimisation of Indole-3-Butyric Acid (IBA) concentrations for production of biomass and phenolics from adventitious roots of Justicia Gendarussa. In: International Conference on Natural Product (ICNP) 2015, 24-25 Mar, 2015, Johor Bahru, Johor.

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Abstract

We investigated different concentrations of lBA to determine its effect on biomass increase and the accumulation of total phenols and flavonoids in adventitious roots of Justiciu gendarussa. Leaf extracts were cultured in MS-based medium supplemented with different concentrations of lBA (2. 3 or 5 mgl." ). These roots were cultured under darkness in shake flasks for 5 weeks. Total phenolic and t1avonoids contents were determined using colorimetric method. Gallic acid and catechin were used as standards. Among the different concentrations of lBA. 2 mgl.' lEA was proven as the best concentration 1';1' adventitious roots biomass production (5.90 g of fresh weight (FW) and 0.53 g of dry weight (OW)). On contrary. 3 mgl," lBA induced higher phenolic and l1avonoid contents than other treatments ( 16.68 rug GAE/g OW and 10.72 mg CTE/g OW). The result indicates that. different lBA concentrations affects biomass and the accumulation of phenolics from adventitious root cultures of.J. gendarussu. Our finding revealed that. lEA was determined the most suitable aux in for adventitious root proliferation of.J. gendurussa . Therefore. the optimisation of auxin concentrations is beneficial to large scale production of biomass and secondary metabolites in 1. gendarussa.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Justicia Gendarussa, gallic acid
Subjects:T Technology > TP Chemical technology
Divisions:Biosciences and Medical Engineering
ID Code:61258
Deposited By: Widya Wahid
Deposited On:31 Mar 2017 06:23
Last Modified:13 Aug 2017 03:28

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