Preparation of zingiber officinale loaded nanostructured lipid carrier (NLC) using ultrasonication and microfluidizer: a comparison

Abstract

Nanostructured lipid cemer (NLC) is the blend of solid lipid. liquid lipid and suitable surfactant foe the purpose of encapsulated poorly water soluble drugs. Zingiber officinale (ginger) extract has been discovered to have anti-oxidant,anti-inflammatory. and anti-microbial effects to the human body. The aim of this study is to develop NLC formulation for encapsulation of Zingiber officinale (ZO) oil and to compare the charecteristic of NLC prepared using different. methods, ultrasonication and microfluidizer. ln this work, the prepared ZO-NLCs were evaluated in terms of particle size zeta potential, entrapment efficiency and drug loading capacity. The morphological study was performed by using Zetasizer Nano S and entrapment efficiency analysis of NLC was performed using HPLC by detecting (6}-gingerol as active biomarker. Based on the study, NLC prepared by ultrasonication and microfluidizer has an evereqe particle size of 100-250 nm and So-l OO nm, respectively, for same formulation. NLC prepared using mlcrofluidirer also exhibit superior entrapment efficiency and drug loading capacity compared to ultrasonication. Based on the analyses, both ultrasonication and microfluidizer method able to prepare NLC for ZO encapsulation, and microfluidizer appeared to have the pctential to produce more stable and desirable properties of NLC.