Trends in characterizing single cell's stiffness properties

Abstract

Biophysical properties of living cells such as mechanical and chemical have been proven to play important roles in regulations of various biological activities including disease progression both at the cellular and molecular levels. In the past decades, a number of research tools have been developed to provide better understanding towards cell¿s biophysical states. This growing interest was supported by the emergence of researches focusing on single cell analysis (SCA) which serves as a platform enabling various experimentation works to be carried out. In this context, various techniques have been developed for single cell¿s mechanical characterization to improve robustness, accuracy and operational flexibility. The generic solution varies from traditional approach, microelectromechanical system (MEMS) and microfluidic. This paper presents a review of progress and developments in the field of single cell mechanical properties specifically discussing on stiffness characterizations. An analytical comparison of the reviewed solutions is presented, and the advantages and disadvantages of different techniques are compared.