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Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli

Mienda, B. S. and Shamsir, M. S. (2015) Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli. Journal Of Biomolecular Structure & Dymics, 33 . pp. 98-100. ISSN 7351-933

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Abstract

Succinic acid is considers to be a platform chemical with divergent applications as a precursor for syntheses of commodity and specialty chemicals. Its biobased production could be a green technology when produced by microbial fermentation using Escherichia coli as a chassis host. Metabolic engineering for increased production of succinic acid using glucose as substrate is beset with limitation of NADH availability under anaerobic conditions in E. coli. In the present work, we used the most recent genome scale metabolic model of E. coli iJ01366 (Orth et al. 2011) to predict metabolic gene knockout of mdh/b3236 with the aim of increasing NADH availability using the glucose substrate.

Item Type:Article
Uncontrolled Keywords:succinic acid, fermentation, E. coli
Subjects:Q Science > Q Science (General)
Divisions:Biosciences and Medical Engineering
ID Code:58572
Deposited By: Haliza Zainal
Deposited On:04 Dec 2016 04:07
Last Modified:14 Sep 2021 08:40

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