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Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent

Tiong, Belinda and Mohd. Bahari, Zaratulnur and Lee, Nor Sahslin Irwan Shah and Jaafar, Jafariah and Ibrahim, Zaharah and Shahir, Shafinaz (2015) Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent. Sains Malaysia, 44 (2). pp. 233-238. ISSN 1266-039

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Official URL: http://dx.doi.org/10.17576/jsm-2015-4402-10

Abstract

Cyanide is highly toxic to the living organisms as it inhibits respiration system in the cell mitochondria. Cyanide iscommonly used in gold extraction process and its discharge into the environment not only causes pollution but it alsobrings harm to the surrounding population. Chemical treatment is expensive and the use of hazardous compound canexacerbate the problem. Biodegradation offers cheap and safe alternative as it overcomes the problems faced by chemicaltreatment. In this study, indigenous bacteria from mining wastewater were isolated. Cyanide degradation was done viashake flask method. A bacterium, designated W2 was found able to grow in the mining wastewater. 16S rRNAanalysisidentified the strain asPseudomonas pseudoalcaligeneswhich could tolerate up to 39 mg/L cyanide concentration andgrowth was depleted at 52 mg/L. 60% cyanide degradation was achieved in wastewater containing medium. End-productanalysis from high performance liquid chromatography (HPLC) detected formamide implicating the role of cyanidehydratase in cyanide degradation. It can be concluded thatP. pseudoalcaligenesis capable of biodegrading cyanideand its potential in wastewater treatment containing cyanide is feasible.

Item Type:Article
Uncontrolled Keywords:biodegradation, bioremediation, cyanide, mining wastewater, pseudomonas pseudoalcaligenes
Subjects:Q Science > Q Science (General)
Divisions:Biosciences and Medical Engineering
ID Code:58198
Deposited By: Haliza Zainal
Deposited On:04 Dec 2016 04:07
Last Modified:07 Sep 2021 10:46

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