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Evaluation and optimization of microbial cellulose (nata) production using pineapple waste as substract

Ching, Chia Hui and Muhammad, Ida Idayu (2007) Evaluation and optimization of microbial cellulose (nata) production using pineapple waste as substract. In: National Research And Innovation Competition (NRIC) 2007, 2007, Penang, Malaysia.

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Abstract

Microbial cellulose produced by Acetobacter xylinum is a new type of biopolymer. It has several unique properties such as high purity and ultra-fine of fiber network, very high hydrophilic, good mechanical strength, and outstanding shape retention and high crystallinity. This thesis was undertaken to evaluate the production of microbial cellulose in static and shaken culture condition using pineapple waste as the substrate for the fermentation process. Shaken culture technique has been carried out at 80 rpm, 120 rpm and 160 rpm. Optimum condition were fixed at pH 5.0 and temperature of 28°C for all the experiments in this research project. From this research, microbial cellulose formation in shaken culture with additional micro particles at 120 rpm is the most suitable as culture medium and the nata produce is more than 70.23% by weight compared to static culture condition. Optimization process for shaken culture with additional micro particles (glass beads) is the best medium culture. Some water-soluble micro particles were added into medium to enhance the production of cellulose in shaken culture condition. With the presence of micro particles, the nata weight increase to 15.19% compared to shaken culture condition. Statistical analysis on experimental design and numerical procedure is the tool in analyzing the yield. Yeast extract and are the most significant factors affect cellulose production. The best selected formulation for preparing the medium culture are yeast extract 6g, sucrose 20g, bactopepton 1.49, KH2PO4 1.08g dan MgSO4 0.06g and nata yield for 441.175g/l.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Acetobacter xylinum, nata, microbial cellulose, pineapple waste, shaken culture condition
Subjects:T Technology > T Technology (General)
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:5811
Deposited By: Norhani Jusoh
Deposited On:10 Jul 2008 09:20
Last Modified:29 Nov 2012 07:43

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