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In silico deletion of PtsG gene in Escherichia coli genome-scale model predicts increased succinate production from glycerol

Mienda, Bashir Sajo and Shamsir, Mohd. Shahir (2015) In silico deletion of PtsG gene in Escherichia coli genome-scale model predicts increased succinate production from glycerol. Journal of Biomolecular Structure and Dynamics, 33 (11). pp. 2380-2389. ISSN 0739-1102

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Official URL: http://dx.doi.org/10.1080/07391102.2015.1036461

Abstract

Systems metabolic engineering and in silico analyses are necessary to study gene knockout candidate for enhanced succinic acid production by Escherichia coli. Metabolically engineered E. coli has been reported to produce succinate from glucose and glycerol. However, investigation on in silico deletion of ptsG/b1101 gene in E. coli from glycerol using minimization of metabolic adjustment algorithm with the OptFlux software platform has not yet been elucidated. Herein we report what is to our knowledge the first direct predicted increase in succinate production following in silico deletion of the ptsG gene in E. coli GEM from glycerol with the OptFlux software platform. The result indicates that the deletion of this gene in E. coli GEM predicts increased succinate production that is 20% higher than the wild-type control model. Hence, the mutant model maintained a growth rate that is 77% of the wild-type parent model. It was established that knocking out of the ptsG/b1101 gene in E. coli using glucose as substrate enhanced succinate production, but the exact mechanism of this effect is still obscure. This study informs other studies that the deletion of ptsG/b1101 gene in E. coli GEM predicted increased succinate production, enabling a model-driven experimental inquiry and/or novel biological discovery on the underground metabolic role of this gene in E. coli central metabolism in relation to increasing succinate production when glycerol is the substrate

Item Type:Article
Uncontrolled Keywords:genome-scale model, gene deletion, glycerol
Subjects:R Medicine > R Medicine (General)
Divisions:Biosciences and Medical Engineering
ID Code:55766
Deposited By: Fazli Masari
Deposited On:06 Oct 2016 06:01
Last Modified:15 Feb 2017 01:41

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