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Optimization of medium for the production of β-cyclodextrin glucanotransferase using central composite design

Ibrahim, Hanan Moawia and Wan Yusoff, Wan Mokhtar and Abdul Hamid, Aidil and Md. Illias, Rosli and Hassan, Othman and Omar, Othman (2005) Optimization of medium for the production of β-cyclodextrin glucanotransferase using central composite design. Process Biochemistry, 40 . pp. 753-758. ISSN 0032-9592

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Abstract

Production of cyclodextrin glucanotransferase (CGTase) from Bacillus G 1, a new bacterial isolate was optimized in shake-flask cultures using statistical design approach. An efficient fermentation medium producing CGTase with high activity (54.9U/ml) was determined: A 2(5) Central Composite Design (CCD; half fraction) has been chosen to elucidate the combined effect of five process variables; tapioca starch, peptone, yeast extract, magnesium sulphate and potassium phosphate concentration. Concentrations of 4% tapioca starch, 2% peptone, 0.04% magnesium sulphate and 1 % sodium carbonate have been shown to be optimum for the production of CGTase.

Item Type:Article
Uncontrolled Keywords:Cyclodextrin glucanotransferase, CCD, medium optimization
Subjects:T Technology > T Technology (General)
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:5574
Deposited By: Norhani Jusoh
Deposited On:23 May 2008 10:38
Last Modified:01 Jun 2010 15:32

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