Universiti Teknologi Malaysia Institutional Repository

Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM

Ahmad, Mohd. Ridzuan and Nakajima, Masahiro and Kojima, Masaru and Kojima, Seiji and Homma, Michio and Fukuda, Toshio (2012) Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM. IEEE Transactions on Nanotechnology, 11 (2). pp. 298-306. ISSN 1536-125X

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1109/TNANO.2011.2171989

Abstract

We performed single pulses current measurement on single cells using dual nanoprobe through environmental scanning electron microscope nanomanipulator system. The ability to characterize the electrical property of single cells can be used as a novel method for cell viability detection in quantitative and instantaneous manners. The nanoprobe was successfully fabricated using focused ion beam tungsten deposition and etching processes. The characteristics of the nanoprobe were examined from the energy dispersion spectrometry and noise analyses. In this paper, for the first time, the electrical property of single cells under their native condition was presented. In order to apply this method for cell viability detection, two types of cells were used, i.e., dead cells and live cells. The results showed that there is a significant difference on the electrical measurement data between dead and live cells.

Item Type:Article
Uncontrolled Keywords:nanomanipulator system, electrical property, electrical measurement
Subjects:T Technology > TA Engineering (General). Civil engineering (General)
Divisions:Chemical Engineering
ID Code:47110
Deposited By: Narimah Nawil
Deposited On:22 Jun 2015 05:56
Last Modified:31 Mar 2019 08:31

Repository Staff Only: item control page