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Antithrombin protein from hirudinaria manillensis: preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene

Looi, Yat Seong and Abd. Latif, Fazilah and Norbib, Normah and Mohd Said, Nazlan (1997) Antithrombin protein from hirudinaria manillensis: preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene. In: Regional Symposium on Chemical Engineering 1997 in conjunction with 13th Symposium of Malaysian Chemical Engineers, 13-15 October 1997, Hyatt Regency, Johor, Malaysia.

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Abstract

Salivary secretions from leeches have been known to possess anticoagulant activity. Studies have been conducted to determine the amino acid sequences and isolation method of this protein. As most studies involved the European leech, Hirudo medicinalis, little studies have been conducted on Hirudinaria manillensis. With the trend of coronary heart disease and other related phatological diseases began to rise, antithrombin protein has potential in the pharmaceutical field. Thus, it is important for mast production of this protein. Molecular biology techniques have made possible cloning and expression of the genes responsible. However, isolation using chromatographic techniques together with the low yield and low activity of the recombinant antithrombin protein have prompt us to look into other alternative of purification and expression system. The N-terminal amino acid sequences of the antithrombin protein from Malaysian local leech were also studied.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Anticoagulant activity, antithrombin protein, hirudinaria manillensis, Malaysia local leeches, salivary secretions
Subjects:T Technology > T Technology (General)
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:4704
Deposited By: Norhani Jusoh
Deposited On:18 Dec 2007 02:16
Last Modified:01 Jun 2010 03:20

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