Universiti Teknologi Malaysia Institutional Repository

Degradation of millimolar concentration of the herbicide dalapon (2,2-dichloropropionic acid) by rhizobium sp. Isolated from soil

Huyop, Fahrul and Cooper, Ronald (2012) Degradation of millimolar concentration of the herbicide dalapon (2,2-dichloropropionic acid) by rhizobium sp. Isolated from soil. Biotechnology and Biotechnological Equipment, 26 (4). pp. 3106-3112. ISSN 1310-2818

Full text not available from this repository.

Official URL: http://dx.doi.org/10.5504/bbeq.2012.0058

Abstract

The herbicide Dalapon is widely used in agricultural areas and is persistent in ground water. A Rhizobium sp. was able to grow at 0.2 mM 2,2-dichloropropionic acid (2,2DCP), which was 100-fold lower than the concentration of the substrate routinely used. Apparently, no new dehalogenases are required to allow growth on this low concentration of 2,2DCP as judged by electrophoretic mobility of dehalogenase proteins in native-PAGE analysis and protein separation by anion-exchange column chromatography. The kinetic analysis suggested that the known dehalogenases were able to act efficiently on low concentrations of haloalkanoic acids. The amount of each dehalogenase, from cells grown on low substrate concentration was different compared to that seen at 20 mM 2,2DCP due to complex regulatory controls, which respond to the growth environment.

Item Type:Article
Uncontrolled Keywords:Bioresource technology
Subjects:T Technology > TP Chemical technology
Divisions:Biosciences and Bioengineering
ID Code:46774
Deposited By: Haliza Zainal
Deposited On:22 Jun 2015 05:56
Last Modified:19 Sep 2017 04:58

Repository Staff Only: item control page