Razali, Firdausi and Moo-Young, Murray and Scharer, Jeno M. and Glick, Bernard R. (2007) Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches. Journal of Chemical and Natural Resources Engineering, 1 . pp. 22-39. ISSN 1823-5255
- Published Version
Official URL: http://www.fkkksa.utm.my/jcnre/vol%201/vol%201%202...
The attraction of employing thermal induction system in the production of heterologous protein is mainly due to 1) a higher expressed protein titer in comparison to constitutive system, and 2) a cleaner process since no chemical inducing agent is added. However, problems pertaining to high temperature and protein overexpression such as inclusion bodies formations, metabolic overload on the host, stress-related proteolysis on the expressed protein, and enhanced acetic acid secretion are commonly encountered. Several bioprocess approaches that include temperature upshift schemes, feeding strategies, timing of induction, two-stage culture mode, and stabilization of plasmid had been implemented to minimize some of these problems. The approaches are reviewed in this article.
|Uncontrolled Keywords:||Escherichia coli, inclusion bodies, matabolic overload, plasmid instability, proteolysis, thermal induction|
|Subjects:||T Technology > TP Chemical technology|
|Divisions:||Chemical and Natural Resources Engineering (Formerly known)|
|Deposited By:||Norhani Jusoh|
|Deposited On:||06 Dec 2007 09:42|
|Last Modified:||05 Oct 2010 05:26|
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