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Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches

Razali, Firdausi and Moo-Young, Murray and Scharer, Jeno M. and Glick, Bernard R. (2007) Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches. Journal of Chemical and Natural Resources Engineering, 1 . pp. 22-39. ISSN 1823-5255

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Abstract

The attraction of employing thermal induction system in the production of heterologous protein is mainly due to 1) a higher expressed protein titer in comparison to constitutive system, and 2) a cleaner process since no chemical inducing agent is added. However, problems pertaining to high temperature and protein overexpression such as inclusion bodies formations, metabolic overload on the host, stress-related proteolysis on the expressed protein, and enhanced acetic acid secretion are commonly encountered. Several bioprocess approaches that include temperature upshift schemes, feeding strategies, timing of induction, two-stage culture mode, and stabilization of plasmid had been implemented to minimize some of these problems. The approaches are reviewed in this article.

Item Type:Article
Uncontrolled Keywords:Escherichia coli, inclusion bodies, matabolic overload, plasmid instability, proteolysis, thermal induction
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:4633
Deposited By: Norhani Jusoh
Deposited On:06 Dec 2007 09:42
Last Modified:15 Jan 2014 00:55

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