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Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semi-defined medium.

El Enshasy, H. and Then, C. and Othman, N. Z. and Al Homosany, H. and Sabry, M. and Sarmidi, M. R. and Aziz, R. A. (2011) Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semi-defined medium. African Journal of Biotechnology, 10 (6). pp. 1029-1038. ISSN 1684-5315

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Abstract

Xanthan gum, a heteropolysaccharide produced by Xanthomonas campasteris, is a widely used biopolymer in food industries. The production process is highly influenced by the type and concentration of the different carbon and nitrogen source as well as other medium components. The aim of this work was to develop an economic industrial medium for industrial production. In shake flask level, the maximal production of xanthan of 19.9 gL-1 was achieved by excluding ammonium nitrate from the industrial medium and making new medium formulation composed of (sucrose, soybean meal, ammonium phosphate and magnesium sulphate). Further optimization in the production process was achieved by transferring the process to 16-L bioreactor and cultivation under controlled pH condition. The maximal volumetric and specific xanthan production [YP/X] obtained were 28 gL-1 and 11.06 g g-1 , respectively. Thus, the semi-defined medium formulation developed in this work could be better and alternatively used for large scale production process for xanthan production when compared to other published media in respect to yield and cost.

Item Type:Article
Uncontrolled Keywords:xanthan production
Subjects:Q Science > QK Botany
Divisions:Others
ID Code:44902
Deposited By: Haliza Zainal
Deposited On:21 Apr 2015 03:31
Last Modified:18 Sep 2017 00:32

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