Universiti Teknologi Malaysia Institutional Repository

High sensitivity differential scannning calorimeter (HSDSC) technique for assaying ginger oleoresin

Morad, Norazian and Abd. Aziz, Mustafa Kamal (2006) High sensitivity differential scannning calorimeter (HSDSC) technique for assaying ginger oleoresin. Project Report. Centre of Lipids Engineering And Applied Research (CLEAR), Universiti Teknologi Malaysia, Kuala Lumpur. (Unpublished)

[img] PDF (Full text)
Restricted to Repository staff only

3844Kb
[img]
Preview
PDF
3359Kb

Abstract

Thermal analysis that is DSC ‘heat-flux’ type was studied as additional or supportive technique to the analytical technique commonly used such as chromatographic, spectroscopic and combination of chromatographic-spectroscopic. Two medicinal plants of Zingiberaceae, that is Zingiber officinale Roscoe (ginger) and Zingiber zerumbet Smith (wild ginger) were investigated. The dried rhizomes of ginger were extracted using solvent extraction techniques to produce crude product (oleoresin) while the fresh rhizomes of wild ginger were extracted using hydrodistillation to produce the essential oil. The major compounds were separated and purified by means of several chromatographic techniques including vacuum column chromatography, gravity column chromatography and preparative thin layer chromatography. The structures were elucidated using spectroscopic methods including 1 H NMR, 13C NMR, IR, GC and GC-MS. Two pure compounds were isolated from Z. officinale oleoresin and identified as 6-gingerol (brownish viscous liquid) and 6-shogaol (yellowish viscous liquid), while zerumbone (white crystals) was isolated from Z. zerumbet essential oil. The crude product as well as the pure compounds were analysed by using DSC technique. This method can be used to identify the major compound in crude product based on qualitative investigation of thermal behaviour (melting point, Tm and heat of fusion, ?Hf ). DSC can also estimate the purity of a substance based on the shape and temperature of the DSC melting endotherm. Sharp peak indicated pure compound while broad peak showed a mixture of various compounds. ABSTRAK (in Malay) Analisis terma iaitu DSC jenis ‘heat-flux’ adalah dikaji sebagai teknik tambahan atau sokongan di samping teknik-teknik analisis yang selalu digunakan seperti kromatografi, spektroskopi dan gabungan kromatografi-spektroskopi. Dua spesies Zingiberaceae iaitu Zingiber officinale Roscoe dan Zingiber zerumbet Smith telah dikaji. Pengekstrakan menggunakan pelarut dijalankan ke atas rizom halia kering bagi mendapatkan ekstak mentah (oleoresin), manakala pengekstrakan rizom segar dilakukan dengan menggunakan teknik penyulingan hidro bagi menghasilkan minyak pati. Pemisahan dan penulenan sebatian kimia utama dilakukan dengan menggunakan pelbagai teknik kromatografi, antaranya kromatografi turus vakum, kromotografi turus graviti dan kromatografi lapisan nipis penyediaan. Sebatian tulen yang berjaya dipisahkan telah dikenal pasti strukturnya melalui pelbagai teknik spektroskopi iaitu RMN 1 H, RMN 13C, IM, KG dan KG-SJ. Dua sebatian tulen utama telah berjaya dipisahkan daripada Z. officinale iaitu 6-gingerol (cecair pekat kuning gelap) dan 6-shogaol (cecair pekat kuning muda), manakala zerumbone (kristal putih) adalah sebatian utama di dalam minyak pati dan ekstrak mentah daripada Z. zerumbet. Ekstrak mentah dan sebatian-sebatian tulen tersebut dianalisa menggunakan teknik DSC. Teknik ini dapat mengenalpasti sebatian utama di dalam ekstrak mentah berdasarkan sifat-sifat termanya (takat lebur, Tm dan perubahan entalpi, ?Hf ). DSC juga mampu menganggarkan ketulenan sesuatu bahan berdasarkan keadaan bentuk dan suhu takat lebut puncak endotermik tersebut. Puncak tajam menunjukan sebatian tulen manakala puncak lebar menunjukan campuran sebatiansebatian utama.

Item Type:Monograph (Project Report)
Uncontrolled Keywords:High Sensitivity Differential Scanning Calorimeter (HSDSC), ginger oleoresin
Subjects:T Technology > TP Chemical technology
Divisions:Others
ID Code:440
Deposited By: Zalina Pareng
Deposited On:29 Nov 2011 01:41
Last Modified:21 Jun 2013 05:55

Repository Staff Only: item control page