Enantioseparation of enilconazole using cyclodextrin-modified MEKC

Abstract

Enantiomeric separation of enilconazole has been successfully achieved using cyclodextrin modified micellar electrokinetic chromatography (CD-MEKC) method with HP-γ-CD as chiral selector. Two parameters which were HP-γ-CD concentration and SDS concentration have been optimized by investigating their effects on resolution and analysis time. Enantioresolution of enilconazole was achieved by the optimized CD-MEKC system containing 40 mM HP-γ-CD, 40mM SDS and 20mM phosphate buffer (pH8) with resolution (Rs=1.3) and analysis time within 9min. Good repeatabilities were obtained in term of RSD% (n=3) for migration time, peak area and peak height ranging from 0.07 to 6.1%. The optimized CD-MEKC method was a simple, less solvent used, and able to perform enantiomeric separation of enilconazole with a good resolution and relatively short analysis time.