Adon, Mohamad Nazib and Dalimin, M. Noh and Abdul Jamil, Muhammad Mahadi and Mohd. Kassim, Norazan (2012) Development of high voltage pulse inducement method for biological cell. In: 2012 International Conference on Biomedical Engineering, ICoBE 2012. IEEE, New York, pp. 501-503. ISBN 978-145771989-9
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Official URL: http://dx.doi.org/10.1109/ICoBE.2012.6179068
Abstract
Electroporation (EP) system is a process of controlling cell functions by using electromagnetic fields (EMF) to create pores through a cellular membrane that causes cell lysis and apoptosis. In this paper we present an experimental setup for fundamental studies on cell EP. An adjustable high voltage pulse generator (3kV/10s-600s pulse length) system were connected to the EP chamber which subsequently allow real time observation of membrane permeability changes and cellular physiology. In order to initiate higher cell viability rate, high transfection efficiency, lower sample contamination and smaller Joule heating the modification of EP chamber need to be implemented Following that, HeLa cell culture has been projected as cell that will be used in this study. Finally, some suggestions are proposed for the future studies.
| Item Type: | Book Section |
|---|---|
| Additional Information: | Indexed by Scopus |
| Uncontrolled Keywords: | electromagnetic field, electroporation, high voltage pulse, real time monitoring |
| Subjects: | T Technology > TK Electrical engineering. Electronics Nuclear engineering |
| Divisions: | Electrical Engineering |
| ID Code: | 35238 |
| Deposited By: | INVALID USER |
| Deposited On: | 30 Oct 2013 00:49 |
| Last Modified: | 02 Feb 2017 04:54 |
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