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Immobilization Of Glucose Oxidase And Ferrocene Redox Polymer In Cross-Linked Poly (Vinyl Alcohol) With Bovine Serum Albumin As Protein Stabilizer

JUSOH, NORHANA and ABDUL-AZIZ, AZILA (2006) Immobilization Of Glucose Oxidase And Ferrocene Redox Polymer In Cross-Linked Poly (Vinyl Alcohol) With Bovine Serum Albumin As Protein Stabilizer. In: Regional Postgraduate Conference On Engineering and Science (RPCES) 2006, 26 -27 July 2006, Johor Bahru, Johor.

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Abstract

A method of tethering a mediator to an enzymatic membrane was studied. Glucose oxidase (GOD) and ferrocene redox polymer were immobilized in cross-linked poly (vinyl alcohol) (CLPVA) with bovine serum albumin (BSA) added as a protein stabilizer. Redox hydrogel polyallylamine ferrocene was prepared by cross-linking polyallylamine hydrochloride with glutaraldehyde and attaching the ferrocene covalently. The biosensor response to glucose was evaluated amperometrically at 0.363V. High BSA concentration resulted in improved current response but lower Kmapp. CLPVA, which had been proven to be an excellent retainer of GOD could not retain both GOD and ferrocene redox polymer effectively, thus affecting the stability of the enzymatic membrane.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Biosensor, Glucose Oxidase, Redox Polymer, Cross-linked PVA, Amperometric.
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:3065
Deposited By: Mohd Nazlee Faisal
Deposited On:17 May 2007 06:21
Last Modified:01 Jun 2010 03:07

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