Wong, Fui Ling and Abdul-Aziz, Azila (2004) Immobilization of Glucose Oxidase on Poly(vinyl alcohol): The Effect of Immobilization Temperature on Apparent Enzyme Activity. In: National Postgraduate Colloquium, 8-9 December 2004, The Gurney Resort Hotel & Residences, Penang.
Amperometric enzymatic glucose biosensors based on glucose oxidase (GOD) are the most widely used devices for continuous regulation of diabeticsâ€™ blood glucose levels. An effective glucose biosensor must contain an immobilized enzyme layer which is stable and is active for a sufficiently long time. For GOD immobilized on poly(vinyl alcohol) through chemical cross-linking, temperature plays an important role on the effectiveness of the cross-linking process. The higher the temperature, the faster the cross-linking process. In an attempt to minimize the possible denaturation of enzyme due to the exposure to the cross-linking temperature, the possibility of immobilizing GOD onto PVA at a lower temperature was investigated. For a hydrogel, membrane water content is generally a good indicator of the cross-linking density of a particular membrane. Thus, the performances of PVA-GOD membranes immobilized at either 4Â°C or 25Â°C but having similar water content were studied. However, no significant differences in the enzyme activity of both types of membranes were observed.
|Item Type:||Conference or Workshop Item (Paper)|
|Uncontrolled Keywords:||Glucose Oxidase, Poly(vinyl alcohol), Temperature, Water content|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Divisions:||Chemical and Natural Resources Engineering (Formerly known)|
|Deposited By:||Mohd Nazlee Faisal|
|Deposited On:||17 May 2007 06:14|
|Last Modified:||01 Jun 2010 03:07|
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