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Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS)

Yap, Wei Ney and Abdul-Rahman, Badarulhisam and Abdul-Aziz, Azila (2004) Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS). In: National Postgraduate Colloquium, 8-9 December 2004, The Gurnay Resort Hotel & Residence, Penang.

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Abstract

An experiment was carried out to study the fundamental factors that affect the growth of Spodoptera frugiperda Sf-9 insect cells. Initial cell density, spent medium carry over and inoculum phase withdrawal significantly influenced the growth kinetics of Sf-9 cells. The percentage of cells infected with Autographa californica multiply-enveloped Nuclear Polyhedrosis Virus (AcMNPV) was obviously affected by spent medium carry over. On the other hand, initial cell density and Multiplicities of Infection (MOI) have minimal influence on infectivity percentage.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Spodoptera frugiperda (Sf-9), Multiplicities of Infection (MOI), Autographa californica multiply-enveloped Nuclear Polydedrosis Virus (AcMNPV)
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:3063
Deposited By: Mohd Nazlee Faisal
Deposited On:17 May 2007 06:06
Last Modified:01 Jun 2010 03:07

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