Isolation and cloning of human VEGF promoter region in PGL3 basic vector

Abstract

Worldwide mortality and morbidity from infectious diseases is being replaced by non infectious chronic diseases, such as cancer, obesity, type II diabetes, cardiovascular diseases, neurodegenerative diseases and aging which may involve inflammation. Vascular endothelial growth factor (VEGF) as a potent pro-inflammatory cytokine is elevated in many human diseases, or animal models of human disease, which are mentioned above. Compounds derived from botanic sources, such as polyphenolic compounds express anti-inflammatory activity by modulation of pro-inflammatory gene expression. We hypothesized this effect may related to control regulation of VEGF gene promoter. In this study, the VEGF promoter was isolated using nested PCR to define the transcription factors binding sites. The human VEGF promoter region was cloned in DH5 alpha Ecoli for future investigation.