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Isolation, screening and characterization of soluble exopolymer-producing bacteria for enhanced oil recovery

Ibrahim, Zaharah and Md. Salleh, Madihah and Yahya, Adibah (2006) Isolation, screening and characterization of soluble exopolymer-producing bacteria for enhanced oil recovery. Regional Postgraduate Conference on Engineering and Science .

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Abstract

Various types of bacteria isolated from samples of water-oil and palm oil mill effluents (POME) were screened for soluble exopolymers which has potential use in microbial enhanced oil recovery (MEOR) applications. The samples were collected from several Malaysian oil reservoirs and Sedenak palm oil mill. Based on the chemical analyses of the water-oil samples, 6 types of media (HAA, HAG, RAA, RAG, MMS and RGM1) were chosen to initiate bacterial growth. Amongst the 52 pure cultures obtained (44 strains from water-oil samples and 8 strains from POME), 19 strains were stable and able to grow rapidly within 24 hours. These strains were further grown in MM medium in order to enhance exopolymer production. Of these, 5 strains were selected based on the presence of slimy colonies and solubility of the exopolymers produced by the bacteria; T221a exhibited the highest production of soluble exopolymers and was the most heat stable strain. T221a was identified as Bacillus licheniformis via biochemical tests and 16S rDNA analyses.

Item Type:Article
Uncontrolled Keywords:Microbial Enhanced Oil Recovery (MEOR), soluble exopolymer-producing bacteria, bacillus licheniformis
Subjects:Q Science > Q Science (General)
Divisions:Science
ID Code:3042
Deposited By: Mr Norahim Ibrahim
Deposited On:17 May 2007 02:02
Last Modified:10 May 2011 09:21

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