Hussin, Huszalina and Hsu, W .C. and Nebenfuhr, M. and Papantoniou, C. (2012) Alkaline Phosphatase Purification from Bacillus megaterium. Kustem Journal .
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Abstract
The aim of this study was to isolate and purify an alkaline phosphatase (ALPs) from Bacillus megaterium. The purified enzyme was established by gel filtration chromatography and high performance liquid chromatography (HPLC). Then, followed by non-denaturing gel electrophoresis to separate the ALPs from described purification technique. The purified enzyme had the following properties: ALPs concentration obtained from enzyme assay culture supernatant was 13.453ïg and selected purification techniques of gel filtration and HPLC were both 19.74ïg and 23.52ïg respectively. The enzyme’s activity and specific activity from culture supernatant were both 0.493 U/ml and 36.3 U/mg respectively.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Bacillus megaterium, alkaline phosphatase, HPLC, non-denaturing gel electrophoresis, gel filtration |
| Subjects: | Q Science > QR Microbiology > QR355 Virology |
| Divisions: | Science |
| ID Code: | 3041 |
| Deposited By: | Norahim Ibrahim |
| Deposited On: | 17 May 2007 00:50 |
| Last Modified: | 19 Oct 2017 05:29 |
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