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Screening of factors influencing pullulan production by aureobasidium melanogenum dsm 2404 using fractional factorial design

Wong, Selly Chew Key and Dailin, Daniel Joe and Mohd. Izwan Low, Luo Zaini and Abd. Malek, Roslinda and El-Enshasy, Hesham Ali (2020) Screening of factors influencing pullulan production by aureobasidium melanogenum dsm 2404 using fractional factorial design. Journal of Critical Review, 7 (18). pp. 3198-3205. ISSN 2394-5125

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Abstract

Pullulan is a water-soluble exopolysaccharide that is synthesized by the black yeast-like-fungus, Aureobasidium sp. Total seven factors including maltose, yeast extract, (NH4)2SO4, peptone, KH2PO4, MgSO4.7H2O, and NaCl were investigated for their effects on pullulan production in shake-flasks by A. melanogenum DSM 2404 using a two-level fractional factorial design (Plackett–Burman Design). The result of variance analysis (ANOVA) proposed that there were four statistically significant (P < 0.05) factors in the production of pullulan by this strain. Maltose, yeast extract, peptone, and KH2PO4 were found to have significant effects on pullulan production using the Plackett–Burman Design. The statistical analysis shows that the linear mathematical model is significant with the coefficient of determination (R2) value of 0.8945.

Item Type:Article
Uncontrolled Keywords:Aureobasidium melanogenum, pullulan, exopolysaccharide, fractional-factorial design
Subjects:Q Science > Q Science (General)
T Technology > TP Chemical technology
Divisions:Chemical and Energy Engineering
ID Code:29680
Deposited By: Yanti Mohd Shah
Deposited On:21 Mar 2013 08:47
Last Modified:31 Jan 2022 08:41

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