Wong, Selly Chew Key and Dailin, Daniel Joe and Mohd. Izwan Low, Luo Zaini and Abd. Malek, Roslinda and El-Enshasy, Hesham Ali (2020) Screening of factors influencing pullulan production by aureobasidium melanogenum dsm 2404 using fractional factorial design. Journal of Critical Review, 7 (18). pp. 3198-3205. ISSN 2394-5125
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Abstract
Pullulan is a water-soluble exopolysaccharide that is synthesized by the black yeast-like-fungus, Aureobasidium sp. Total seven factors including maltose, yeast extract, (NH4)2SO4, peptone, KH2PO4, MgSO4.7H2O, and NaCl were investigated for their effects on pullulan production in shake-flasks by A. melanogenum DSM 2404 using a two-level fractional factorial design (Plackett–Burman Design). The result of variance analysis (ANOVA) proposed that there were four statistically significant (P < 0.05) factors in the production of pullulan by this strain. Maltose, yeast extract, peptone, and KH2PO4 were found to have significant effects on pullulan production using the Plackett–Burman Design. The statistical analysis shows that the linear mathematical model is significant with the coefficient of determination (R2) value of 0.8945.
Item Type: | Article |
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Uncontrolled Keywords: | Aureobasidium melanogenum, pullulan, exopolysaccharide, fractional-factorial design |
Subjects: | Q Science > Q Science (General) T Technology > TP Chemical technology |
Divisions: | Chemical and Energy Engineering |
ID Code: | 29680 |
Deposited By: | Yanti Mohd Shah |
Deposited On: | 21 Mar 2013 08:47 |
Last Modified: | 31 Jan 2022 08:41 |
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