Mohd. Muhid, Mohd. Nazlan and Hamdan, Halimaton and Sani, S. (2011) Design, synthesis and activity study of tyrosinase encapsulated silica aerogel (TESA) biosensor for phenol removal in aqueous solution. Journal of Sol-Gel Science and Technology, 59 (1). pp. 7-18. ISSN 0928-0707
Official URL: http://dx.doi.org/10.1007/s10971-011-2454-3
Tyrosinase encapsulated silica aerogel (TESA) was synthesized via an alcohol-free colloidal sol–gel route at room temperature and at neutral pH. Characterization on TESA indicated that 98% of enzyme was effectively loaded and located inside the aerogel network. TESA without solvent extraction showed higher tyrosinase activity than TESA extracted by amyl acetate/acetone (v/v:1/1). Stability of tyrosinase in TESA was enhanced towards extreme temperature, acidic and basic conditions. Optimization study indicates that 500 U enzyme/g silica aerogel; aged for 2 days, showed superior performance in the oxidation of catechol. The activity of TESA was remarkably enhanced; which was active at a wider temperature (up to 80 °C) and pH range (4–9). In contrast, free tyrosinase was totally inactive at these pH values and temperature >55 °C. TESA successfully removed about 90% of phenol in aqueous solution after 3 h of contact time with excellent reusability.
|Uncontrolled Keywords:||silica aerogel, encapsulation, enzyme, tyrosinase, phenol|
|Deposited By:||Liza Porijo|
|Deposited On:||30 Nov 2012 00:47|
|Last Modified:||30 Nov 2012 00:47|
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