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Design, synthesis and activity study of tyrosinase encapsulated silica aerogel (TESA) biosensor for phenol removal in aqueous solution

Mohd. Muhid, Mohd. Nazlan and Hamdan, Halimaton and Sani, S. (2011) Design, synthesis and activity study of tyrosinase encapsulated silica aerogel (TESA) biosensor for phenol removal in aqueous solution. Journal of Sol-Gel Science and Technology, 59 (1). pp. 7-18. ISSN 0928-0707

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Official URL: http://dx.doi.org/10.1007/s10971-011-2454-3

Abstract

Tyrosinase encapsulated silica aerogel (TESA) was synthesized via an alcohol-free colloidal sol–gel route at room temperature and at neutral pH. Characterization on TESA indicated that 98% of enzyme was effectively loaded and located inside the aerogel network. TESA without solvent extraction showed higher tyrosinase activity than TESA extracted by amyl acetate/acetone (v/v:1/1). Stability of tyrosinase in TESA was enhanced towards extreme temperature, acidic and basic conditions. Optimization study indicates that 500 U enzyme/g silica aerogel; aged for 2 days, showed superior performance in the oxidation of catechol. The activity of TESA was remarkably enhanced; which was active at a wider temperature (up to 80 °C) and pH range (4–9). In contrast, free tyrosinase was totally inactive at these pH values and temperature >55 °C. TESA successfully removed about 90% of phenol in aqueous solution after 3 h of contact time with excellent reusability.

Item Type:Article
Uncontrolled Keywords:silica aerogel, encapsulation, enzyme, tyrosinase, phenol
Subjects:Q Science
Divisions:Science
ID Code:28875
Deposited By: Liza Porijo
Deposited On:30 Nov 2012 00:47
Last Modified:30 Nov 2012 00:47

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