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Catalytic activity and stability of laccase entrapped in sol-gel silica with additives

Mohidem, N. A. and Mat, Hanapi (2011) Catalytic activity and stability of laccase entrapped in sol-gel silica with additives. Journal of Sol-Gel Science and Technology, 61 (1). pp. 1-8. ISSN 0928-0707

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Official URL: http://dx.doi.org/10.1007/s10971-011-2596-3


This study investigated the effects of different additives and precursors on the catalytic activity of laccase entrapped in sol-gel silica. It was found that the laccase catalytic activity and stability of sol-gel laccase could be enhanced if the entrapment was performed in the presence of additives such as PVA, PEG and APTS. The use of TEOS as a precursor showed slightly higher laccase catalytic activity compared to TMOS. The PVA as an additive showed a better catalytic activity enhancement compared to the PEG and APTMS with the optimum PVA concentration of 0.03 mg/mL. The optimal temperatures of sol-gel laccase without and with additives were found to be at 40 and 27°C, respectively. After 70 days of storage at 27°C, the catalytic activity of the immobilized sol-gel laccase with additives maintained its catalytic activity compared to only 30% of its original catalytic activity for the sol-gel laccase without additives.

Item Type:Article
Uncontrolled Keywords:additives, catalytic activity, entrapment, laccase, sol-gel silica, stability
Subjects:Q Science > QD Chemistry
Divisions:Chemical Engineering
ID Code:28831
Deposited By: Mrs Liza Porijo
Deposited On:29 Nov 2012 06:53
Last Modified:13 Feb 2017 03:17

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