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Development and modification of PVA-alginate as a suitable immobilization matrix

Mohd. Zain, Nor Azimah and Suhaimi, M. S. and Idris, Ani (2011) Development and modification of PVA-alginate as a suitable immobilization matrix. Process Biochemistry, 46 (11). pp. 2122-2129. ISSN 1359-5113

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Official URL: http://dx.doi.org/10.1016/j.procbio.2011.08.010

Abstract

This study reports the immobilization of invertase in the PVA-alginate matrix via a modified method. The PVA-alginate modified method involved the treatment of immobilized beads in sodium sulfate solution. In this study, the steps involved in the preparation of matrix were analyzed. The influence of the sodium sulfate concentration on the bead formation was investigated and its mechanism of immobilization was further elucidated by means of FTIR and EDX. In addition, the stability of invertase in sodium sulfate and boric acid solution was also studied. EDX results revealed that borates were completely replaced by sulfates as the cross-linking agent. Through the mechanism of nucleophilic substitution, it was revealed that sulfate attacked the carbon atom bearing borate on the opposite side to the side bonded to borate due to steric hindrance. Carbon therefore underwent backside attack by sulfate and inverted its original configuration through a phenomenon known as Walden Inversion. The presence of sulfate was also supported by FTIR spectra. The best sodium sulfate concentration was found to be 0.5 M.

Item Type:Article
Uncontrolled Keywords:EDX, FTIR, Invertase, PVA-alginate beads, sodium sulfate, walden inversion
Subjects:Q Science
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:28795
Deposited By: Liza Porijo
Deposited On:29 Nov 2012 02:54
Last Modified:29 Nov 2012 02:54

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