Basic in vitro studies of human skin culture: melastoma malabathricum extract on fibroblasts growth

Abstract

The objectives of this project were to isolate two types of cell, keratinocytes and fibroblasts from human skin, study the cells growth and to test the effect of Melastoma malabathricum extract on these two cell lines. MTT assay was used to test cell viability in the experiments which carried out in 96 wells plate. Keratinocytes and fibroblasts were successfully isolated and maintain in cultures. Keratinocytes show differentiation after 2 or 3 passages. Fibroblasts culture show better proliferation than keratinocytes culture. The study found that high seeding density (more than 20000 cells/cm2) suitable for keratinocytes and low seeding density (less than 10000 cells/cm2) suitable for fibroblasts. Extract of Melastoma malabathricum shown to be not effected fibroblasts attachment. Fibroblast cells are not shown any toxic effect at concentration below 10ug/ml. However at concentration ranging from 10 g/ml to 1000 g/ml, the cells relative viability decreased (93.4% to 63.9%). Keratinocytes were not used to test with the extracts since cells start differentiated only after 2 or 3 passages.