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Molecular Identification of Candida Tropicalis RETL-Cr1 By PCR Amplification of Ribosomal DNA

Husin , Aishah and Mohd. Tuah, Piakong and Abdul Rashid, Noor Aini and Yahya, Adibah and Md. Salleh, Madihah and Syed Mohd. Rafeii, Sharifah Norhafizah (2004) Molecular Identification of Candida Tropicalis RETL-Cr1 By PCR Amplification of Ribosomal DNA. Jurnal Sains dan Teknologi, 15 . pp. 15-22. ISSN 2231-9085

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Abstract

PCR amplification of ribosomal DNA was used to identify a phenol-degrading yeast strain. Theinternal transcribed spacer regions of the yeast was amplified using universal primers ITSI and ITS4. Based on a BLASTN search of genBank, the complete sequences of ITSI-%.*S rDNA-ITS2 regions and portions of 18S and 28S for the purified DNA products of RETL-Crl shared 98% similarity with Candida tropicalis. The nucleotide swquences of the C. tropicalis RETL-Crl was submitted to the GenBank database under the accession number AY725426

Item Type:Article
Uncontrolled Keywords:Candida tropicalis, per-age, phenol-degrading yeast
Subjects:Q Science > Q Science (General)
Divisions:Others
ID Code:28193
Deposited By: Nurul Asilah Mahmood
Deposited On:18 Sep 2012 06:09
Last Modified:02 Oct 2012 06:28

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