Molecular identification of Candida tropicalis RETL-Cr1 By PCR amplification of Ribosomal DNA

Abstract

PCR amplification of ribosomal DNA was used to identify a phenol-degrading yeast strain. Theinternal transcribed spacer regions of the yeast was amplified using universal primers ITSI and ITS4. Based on a BLASTN search of genBank, the complete sequences of ITSI-%.*S rDNA-ITS2 regions and portions of 18S and 28S for the purified DNA products of RETL-Crl shared 98% similarity with Candida tropicalis. The nucleotide swquences of the C. tropicalis RETL-Crl was submitted to the GenBank database under the accession number AY725426.