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Cloning and expression of pullulanase gene from locally isolated bacillus SP

Nik Mahmood, Nik Azmi and Md. Illias, Rosli and Md. Salleh, Madihah and Hassan, Osman and Kamaruddin, Kamarulzaman and Shahab, Neelam (2006) Cloning and expression of pullulanase gene from locally isolated bacillus SP. Project Report. Universiti Teknologi Malaysia. (Unpublished)

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Official URL: https://www.researchgate.net/publication/254655826...


Bacterial pullulanase represents one of th e starch-degrading enzymes that are widely used in the starch processing indu stry along with amylases. Amylases hydrolyze a -(1,4 )-glycosidic linkage in starch to produce a mixture of glucose , maltooligo sacchari de and limited a-dextrin. All the remaining a -(1,6)-glycosid ic branches in the products are hydrolyzed by p ullulanase. This is an advantage t o improve glucose production by coupling pullulanase and amylase in the p rocess. As such, many pullulana e enzyme has been isolated and one has been showing optimum pH of 10-10.5 which is suitable for use in dishwasher detergent additive in removal of star ch stain. We have recently iso lated a few bacterias that have shown potentially pullulanase producers by the holo-zone in pullulan-plate assay. One of them, we named Bacillus –1 sho ws a bigger holo-zone among others, Bacillus- 1 is highly active in pH more than 7. The enzyme also shows a mo derate activity to wards starch that may be indicates be side hydrolyzes a -(1,6)-glycosidic linkage in starch, it also hydrolyzes a -(1,4)- glycosidi c simi lar to a -amylase. Unfortunately the enzyme from wild-type bacteria is in lower yield an d in this studies, we intend to clone and sequence the pullulanase gene and also expressed the gene in a high expression system to be able to produce in a high yield before characterizing expressed protein.

Item Type:Monograph (Project Report)
Uncontrolled Keywords:Molecular Cloning; Expression; Recombinant pullulanase enzyme
Subjects:Q Science > Q Science (General)
ID Code:2754
Deposited By: Nor Azlin Nordin
Deposited On:05 Jan 2014 07:33
Last Modified:11 Oct 2017 04:29

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