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Suitable culture conditions for growth of primary human skin fibroblast

Abdul Majid, Fadzilah Adibah and Idrus, Ruzymah and Ting, Lee Yu (2008) Suitable culture conditions for growth of primary human skin fibroblast. In: Special topics in bioprocess engineering. Penerbit UTM, Johor, pp. 69-102. ISBN 978-983-52-0602-5

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Official URL: http://www.penerbit.utm.my/bookchapterdoc/FKKKSA/b...

Abstract

Human skin fibroblasts are the major cell type in the dermis for synthesis and reorganization of ECM (extracellular matrix) components during wound repair. In addition, they are capable of secreting factors that regulate the growth and differentiation of other cells [1]. The net proliferative rate of skin varies with the region of the body. The turnover of skin is in the order of a few weeks, which regenerates a large amount of flakes over time. Skin is the body’s third most prolific tissue [2]. Fibroblasts are a well established system for in vitro analysis of cell growth, migration, and collagen metabolism [3]. They have been used to study skin aging [4-5], wound healing [6], genetic disorder [7-8], disease [9], evaluating cosmetic formulations toxicity [10] and chemical cytotoxicity [11-12]. In recent years, the reconstruction of human tissue engineering skin has produced several marketed models, which vary from the simple to the complex system. These skin substitutes composed of autologous epidermal cell sheets (Epicel®, Laserskin®), dermal substrates (Alloderm®, Dermagraft®) and temporary coverings (Transcyte®). In addition, human skin equivalents composed of living epidermis and dermis are now available (Apligraf®, OrCelâ®) [13].

Item Type:Book Section
Uncontrolled Keywords:growth, human skin fibroblast
Subjects:T Technology > T Technology (General)
T Technology > TP Chemical technology
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:26319
Deposited By: Fazli Masari
Deposited On:30 Jul 2012 04:09
Last Modified:30 Jul 2012 04:09

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