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Isolation and identification of radical scavenging and tyrosinase inhibition of polyphenols from tibouchina semidecandra L.

Mohd. Sirat, Hasnah and Rezali, Mohd. Fazlin and Ujang, Zanariah (2010) Isolation and identification of radical scavenging and tyrosinase inhibition of polyphenols from tibouchina semidecandra L. Journal Of Agricultural and Food Chemistry, 58 (19). 10404 - 10409. ISSN 0021-8561

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Official URL: http://dx.doi.org/10.1021/jf102231h

Abstract

Phytochemical and bioactivity studies of the leaves and stem barks of Tibouchina semidecandra L. have been carried out. The ethyl acetate extract of the leaves yielded four flavonoid compounds, identified as quercetin, quercetin 3-O-a-l-(2''-O-acetyl) arabinofuranoside, avicularin, and quercitrin, while the stem barks gave one ellagitannin, identified as 3,3'-O-dimethyl ellagic acid 4-O-a-l-rhamnopyranoside. Evaluation of the antioxidative activity on the crude extracts and pure compounds by electron spin resonance (ESR) and ultraviolet-visible (UV-vis) spectrophotometric assays showed that the pure isolated polyphenols and the EtOAc extract possessed strong antioxidative capabilities. Quercetin was found to be the most active radical scavenger in DPPH-UV and ESR methods with SC(50) values of 0.7 µM ± 1.4 and 0.7 µM ± 0.6 µM, respectively, in the antioxidant assay. A combination of quercetin and quercitrin was tested for synergistic antioxidative capacity;, however, there was no significant improvement observed. Quercetin also exhibited strong antityrosinase activity with a percent inhibition of 95.0% equivalent to the positive control, kojic acid, in the tyrosinase inhibition assay.

Item Type:Article
Uncontrolled Keywords:tibouchina semidecandra L., Melastomataceae, polyphenols, radical scavenging activity, tyrosinase inhibition activity
Subjects:Q Science
Divisions:Science
ID Code:26297
Deposited By: Liza Porijo
Deposited On:29 Jun 2012 07:23
Last Modified:01 Aug 2012 03:29

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