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Nanoindentation methods to measure viscoelastic properties of single cells using sharp, flat, and buckling tips inside ESEM

Ahmad, Mohd. Ridzuan and M., Nakajima and S., Kojima and M., Homma and T., Fukuda (2010) Nanoindentation methods to measure viscoelastic properties of single cells using sharp, flat, and buckling tips inside ESEM. IEEE Transactions on Nanobioscience, 9 (1). p. 40900. ISSN 1536-1241

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Official URL: http://dx.doi.org/10.1109/TNB.2009.2034849

Abstract

In this paper, methods to measure viscoelastic properties of time-dependent materials are proposed using sharp, flat, and buckling tips inside an environmental SEM. Single W303 yeast cells were employed in this study. Each of the tips was used to indent single cells in a nanoindentation test. Three loading histories were used: 1) a ramp loading history, in which a sharp indenter was used; 2) a step loading history, in which a flat indenter was implemented; and 3) a fast unloading history, in which a buckling nanoneedle was applied. Analysis of the viscoelastic properties of single cells was performed for each of the loading histories by choosing an appropriate theory between the correspondence principle and the functional equation. Results from each of the tests show good agreement, from which strong conclusion can be drawn.

Item Type:Article
Uncontrolled Keywords:Environmental-SEM (ESEM), nanomanipulation, single cell analysis, viscoelasticity, yeast cells
Subjects:T Technology > TK Electrical engineering. Electronics Nuclear engineering
Divisions:Electrical Engineering
ID Code:26260
Deposited By: Liza Porijo
Deposited On:29 Jun 2012 03:34
Last Modified:19 Aug 2014 04:07

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