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Purification strategies for recombinant human transferrin expressed using baculovirus expression vector systems

Azila Abd. Aziz, (2006) Purification strategies for recombinant human transferrin expressed using baculovirus expression vector systems. In: Special Topics In Bioprocess Engineering Volume I. Penerbit UTM, Johor , pp. 171-188. ISBN 978-983-52-0600-9

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Abstract

Baculoviruses, which are double stranded, circular, supercoiled DNA molecules in a rod-shaped capsid, are the most well-known viruses that infect the insect world [1]. Most of the baculoviruses are isolated from arthropod, predominantly from the species Lepidoptera. Two isolates which are well known in foreign gene expression are Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) and Bombyx mori nuclear polyhedrosis virus (BmNPV). AcMNPV is usually used to infect cultures and larvae of Spodotera frugiperda (Sf) and Trichoplusia ni (Tn). BmNPV infects silkworm (Bombyx mori) cultures and larvae. Baculovirus Expression Vector System (BEVs) is based on the introduction of a foreign gene of interest to replace the naturally occurring polyhedron gene within the wild type baculovirus genome. Thus, recombinant product is expressed instead of the naturally occurring polyhedron protein in cultured insect cells and insect larvae.

Item Type:Book Section
Subjects:Unspecified
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:25684
Deposited By: Liza Porijo
Deposited On:25 May 2012 04:02
Last Modified:25 May 2012 04:02

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